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What are the common assay methods used in clinical labs?

An example of an AI chatbot conversation about assay methods in clinical laboratories.

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You

What are the common assay methods used in clinical labs?

MagicBuddy
MagicBuddy

Common assay methods in clinical labs include ELISA, PCR, and mass spectrometry. Each has unique applications.

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You

Can you explain how ELISA works?

MagicBuddy
MagicBuddy

ELISA, or Enzyme-Linked Immunosorbent Assay, detects proteins or antibodies using enzyme-linked antibodies and color change.

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You

What are the advantages of using PCR?

MagicBuddy
MagicBuddy

PCR, or Polymerase Chain Reaction, is highly sensitive and can amplify small DNA samples for various analyses.

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You

Are there any limitations to these methods?

MagicBuddy
MagicBuddy

Yes, limitations include sensitivity to contamination in PCR and potential cross-reactivity in ELISA.

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You

How do labs choose which method to use?

MagicBuddy
MagicBuddy

Labs consider factors like the type of sample, required sensitivity, specificity, and the purpose of the assay.

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