What are the common assay methods used in clinical labs?
Common assay methods in clinical labs include ELISA, PCR, and mass spectrometry. Each has unique applications.
Can you explain how ELISA works?
ELISA, or Enzyme-Linked Immunosorbent Assay, detects proteins or antibodies using enzyme-linked antibodies and color change.
What are the advantages of using PCR?
PCR, or Polymerase Chain Reaction, is highly sensitive and can amplify small DNA samples for various analyses.
Are there any limitations to these methods?
Yes, limitations include sensitivity to contamination in PCR and potential cross-reactivity in ELISA.
How do labs choose which method to use?
Labs consider factors like the type of sample, required sensitivity, specificity, and the purpose of the assay.
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