What are cytotoxicity assays and how are they performed?
An example of an AI chatbot conversation about cytotoxicity assays and their methodologies.
What are cytotoxicity assays and how are they performed?
Cytotoxicity assays are tests used to determine the toxicity of substances on cells. They help assess the safety of drugs and chemicals.
What are some common types of cytotoxicity assays?
Common types include MTT assay, LDH assay, and Trypan Blue exclusion assay. Each has its own methodology and applications.
How do I choose the right assay for my research?
Choosing the right assay depends on factors like the type of cells, the substance being tested, and the specific research goals.
Are there any limitations to these assays?
Yes, limitations include false positives/negatives, variability in cell types, and the need for specific controls.
Can you explain the MTT assay in detail?
Sure! The MTT assay measures cell viability based on the reduction of MTT to formazan by metabolically active cells.
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