What are enzyme activity assays and how are they performed?

What are enzyme activity assays and how are they performed?

Enzyme activity assays are laboratory methods used to measure the activity of enzymes. They typically involve monitoring the rate of a reaction catalyzed by the enzyme under specific conditions.

What types of enzyme activity assays are there?

There are several types, including continuous assays, endpoint assays, and coupled assays. Each type has its own methodology and applications.

Can you explain how a continuous assay works?

In a continuous assay, the reaction progress is monitored in real-time, often using spectrophotometry to measure changes in absorbance as the reaction proceeds.

What factors can affect enzyme activity during these assays?

Factors include temperature, pH, substrate concentration, and the presence of inhibitors or activators.

How do I choose the right assay for my enzyme?

Choosing the right assay depends on the enzyme's characteristics, the desired sensitivity, and the specific conditions of your experiment.