How can I measure enzyme activity in a lab?
You can measure enzyme activity using various methods, such as spectrophotometry, fluorometry, or colorimetry. Which method are you interested in?
I'm interested in using spectrophotometry. How does that work?
Spectrophotometry measures the absorbance of light by a solution. You can track the change in absorbance over time to determine enzyme activity.
What specific steps should I follow for a spectrophotometric assay?
First, prepare your enzyme and substrate solutions. Then, mix them in a cuvette and measure the absorbance at specific intervals using a spectrophotometer.
Are there any specific wavelengths I should use for different enzymes?
Yes, different enzymes have optimal wavelengths based on the substrate used. It's best to consult literature for specific enzymes.
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