What are the different enzyme assay methods?

What are the different enzyme assay methods?

There are several enzyme assay methods, including spectrophotometric, fluorometric, and colorimetric assays. Each method has its own advantages depending on the enzyme and the substrate used.

Can you explain how a spectrophotometric assay works?

Sure! A spectrophotometric assay measures the absorbance of light by a solution at a specific wavelength. As the enzyme catalyzes a reaction, the concentration of the product changes, which alters the absorbance and allows for quantification.

What are the advantages of using fluorometric assays?

Fluorometric assays are highly sensitive and can detect low concentrations of substrates or products. They also allow for real-time monitoring of enzyme activity.

Are there any limitations to these methods?

Yes, limitations include potential interference from other substances in the sample, the need for specific conditions, and sometimes the requirement for expensive equipment.