What are some common enzyme engineering techniques?
Common techniques include directed evolution, rational design, and site-directed mutagenesis.
Can you explain directed evolution?
Directed evolution mimics natural selection to evolve proteins or nucleic acids toward a user-defined goal.
What is rational design?
Rational design involves using knowledge of enzyme structure and function to make specific changes to improve activity.
How does site-directed mutagenesis work?
Site-directed mutagenesis allows for the precise alteration of specific amino acids in an enzyme to study their effects.
What are the applications of these techniques?
These techniques are used in drug development, biofuels, and industrial biocatalysis.
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