What factors affect enzyme inhibition?

What factors affect enzyme inhibition?

Enzyme inhibition can be affected by several factors including temperature, pH, substrate concentration, and the presence of inhibitors.

Can you explain how temperature affects enzyme activity?

Temperature can influence enzyme activity significantly. Generally, as temperature increases, enzyme activity increases until it reaches an optimal temperature, after which the activity declines due to denaturation.

What are competitive and non-competitive inhibitors?

Competitive inhibitors bind to the active site of the enzyme, preventing substrate binding. Non-competitive inhibitors bind to a different site, altering the enzyme's function without blocking the active site.

How does pH affect enzyme function?

pH affects the ionization of the enzyme and substrate, which can alter the enzyme's shape and function. Each enzyme has an optimal pH range.

What role does substrate concentration play in enzyme inhibition?

At low substrate concentrations, increasing substrate can increase reaction rates. However, at high concentrations, the effect of inhibitors becomes more pronounced.