What are the experimental methods used in enzyme kinetics?
An example of an AI chatbot conversation about experimental methods in enzyme kinetics.
What are the experimental methods used in enzyme kinetics?
Enzyme kinetics often employs methods like initial rate measurements, steady-state kinetics, and rapid kinetics.
Can you explain the initial rate method in more detail?
Sure! The initial rate method measures the rate of reaction at the very beginning, before substrate depletion affects the reaction.
What is steady-state kinetics?
Steady-state kinetics assumes that the concentration of the enzyme-substrate complex remains constant over time during the reaction.
How does rapid kinetics differ from other methods?
Rapid kinetics involves techniques like stopped-flow and flash photolysis to observe fast reactions in real-time.
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