How do you measure enzyme activity in a lab?

How do you measure enzyme activity in a lab?

Enzyme activity can be measured using various methods, such as spectrophotometry, fluorometry, or by monitoring substrate conversion.

What is the most common method used?

Spectrophotometry is one of the most common methods, as it allows for the quantification of product formation by measuring absorbance.

Can you explain how spectrophotometry works?

Sure! In spectrophotometry, a light beam passes through a sample, and the amount of light absorbed by the product is measured, indicating enzyme activity.

What factors can affect enzyme activity?

Factors include temperature, pH, substrate concentration, and the presence of inhibitors or activators.

How do I determine the optimal conditions for an enzyme?

You can perform experiments by varying one factor at a time, such as temperature or pH, and measuring the enzyme activity to find optimal conditions.