How can I troubleshoot enzyme activity in my experiment?

How can I troubleshoot enzyme activity in my experiment?

To troubleshoot enzyme activity, first check the pH and temperature conditions, as they can significantly affect activity.

What if the enzyme is still not working after adjusting pH and temperature?

You might want to verify the enzyme concentration and ensure that the substrate is not limiting. Have you checked those?

Yes, I have the right concentrations. Could there be any inhibitors present?

Absolutely! Contaminants or inhibitors can affect enzyme activity. Consider running a control experiment to identify any inhibitors.

Thanks! How can I identify potential inhibitors in my samples?

You can perform a series of assays with known inhibitors to see if they affect your enzyme's activity, or use chromatography techniques.