How do I use controls in assays?

How do I use controls in assays?

Using controls in assays is crucial for ensuring the accuracy and reliability of your results. Controls help to validate the assay performance.

What types of controls should I include?

You should include positive controls, negative controls, and blank controls. Positive controls confirm that the assay works, negative controls check for contamination, and blanks help to identify background noise.

How do I interpret the results with controls?

Interpretation involves comparing your sample results against the controls. If your sample results are similar to the positive control, the assay is likely valid. If they are similar to the negative control, it may indicate an issue.

Can you give an example of a control setup?

Sure! For a PCR assay, you might set up a positive control with known DNA, a negative control with no DNA, and a blank with just the reagents. This setup helps ensure your assay is functioning correctly.