What is the western blotting technique?

What is the western blotting technique?

The western blotting technique is a method used to detect specific proteins in a sample using gel electrophoresis and antibodies.

How does the process work step by step?

First, proteins are separated by gel electrophoresis, then transferred to a membrane, and finally probed with antibodies.

What are the common applications of western blotting?

Common applications include protein expression analysis, disease diagnosis, and research in molecular biology.

Are there any limitations to this technique?

Yes, limitations include the need for specific antibodies, potential for non-specific binding, and time-consuming protocols.