What are kinetic assays and how are they used in drug development?

What are kinetic assays and how are they used in drug development?

Kinetic assays are experimental methods used to measure the rate of a reaction over time. In drug development, they help assess how a drug interacts with its target.

Can you explain the difference between kinetic and equilibrium assays?

Sure! Kinetic assays measure the rate of a reaction, while equilibrium assays measure the state of a reaction once it has reached balance.

What are some common applications of kinetic assays?

Common applications include enzyme activity measurement, drug binding studies, and assessing the effects of inhibitors in pharmacology.

How do you set up a kinetic assay in the lab?

Setting up a kinetic assay involves selecting the appropriate substrate, enzyme, and conditions, then measuring the reaction over time using techniques like spectrophotometry.

What challenges are faced when conducting kinetic assays?

Challenges include maintaining consistent conditions, accurately measuring reaction rates, and dealing with potential interference from other substances.